Runners will leave Green Park at 10:15, heading to Whitley and then on to the University of Reading campus before making their way towards the town centre.
They will continue to Prospect Park before hitting the A4 Bath Road and on down the A33 to the finish line at the stadium.
More than 15,000 well-wishers are expected to line the route, cheering on experienced runners, fun runners and those raising money for charity.
In 2025, participants raised just under £500,000 for dozens of good causes.
Before the main event, a 3km (1.8 miles) mini marathon will take place in Green Park from 08:30.
